中國醫藥大學課程查詢系統

課程基本資料

系所 / 年級Department/Grade-Level

轉譯醫學暨新藥開發研究所碩士班 1年級

課號 / 班別Course Code

MPM000024 / A

學分數Credits

1 學分

選 / 必修Elective / Required

選修(Elective)

科目中文名稱Course Title (Chinese)

真核生物基因轉錄與表觀調控

科目英文名稱Course Title (English)

Mechanisms of transcriptional regulation and epigenetic control

負責教師Instructor

許凱文(Hsu, Kai-Wen)

開課期間Course Load

一學期

人數上限Enrollment Max.

30 人

已選人數Enrollment Taken

2 人

抽籤自動遞補等候人數Number of the waiting list after ballot

0 人

備註Memo

本課程依據國外學者至本校短期交流期間開設系列課程)(English course (Depend on Prof. Cheng-Ming Chiang arrive to CMU) 全英語授課(Full English course)

可選學制 (availability)

大學部

不可選

二技部

不可選

碩士班

1年級至4年級

博士班

不可選

修讀他所『碩士班可供博士班下修課程』是否認列為博士班畢業學分，請先向所屬研究所確認，以免日後所修學分不予認列。

請各位同學遵守智慧財產權觀念；請勿非法影印。

教學綱要

課程概述Course Description

1. 基礎轉錄蛋白、轉錄輔因子、與轉錄因子的作用機制 (The mechanism of transcriptional factors) 2. 基因啟動子、增強子、與絕緣子的作用 (Gene promoter, enhancer, and insulators) 3. 染色質絲與表觀調控 (Chromatin and epigenetic regulation) 4. 癌症與病毒基因的轉錄調控 (Cancer and viral gene transcriptional regulation) 備註: 於本所客座教授來台短期交流期間授課，選課前請與本所辦公室聯繫。全英語授課(Full English course)

教學目標Course Objectives

真核細胞的基因轉錄作用(包含各種轉錄蛋白的分子作用機制、表觀基因體的調控、癌症與病毒基因的轉錄機制) Mammalian cells gene transcription (transcriptional factors, epigenetic regulation, Cancer and viral gene transcriptional regulation)

先修科目Prerequisites

生物化學、分子生物學 biochemistry, molecular biology

教學方式Teaching Methods

非同步教學(主要提供線上影音教材，透過線上教學平台進行作業及討論) Asynchronous Online Learning 講課 Lecturing 由學生自訂學習目標與抱負水準 Self-defined learning goal and evaluation standard 學生上台報告、角色演練 Oral presentation, Role playing

評量方式Assessment

期末報告 Final report 個人上台報告 Oral Report 出席狀況 Class attendance 課堂參與與表現 Class involvement 心得與反思報告 Learning gain and introspection report
口頭報告(70%)、出缺席率、上課表現（30％） Oral presentation (70%), class attendance and involvement (30%)

參考書目Reference

Heintzman, N.D. and Ren, B. (2009) Finding distal regulatory elements in the human genome. Curr. Opin. Genet. Dev. 19: 541-549. Hnisz, D., Abraham, B.J., Lee, T.I., Lau, A., Saint-André, V., Sigova, A.A., Hoke, H.A., and Young, R.A. (2013) Super-enhancers in the control of cell identity and disease. Cell 155: 934-947. Wu, S.-Y., Nin, D.S., Simanski, S., Kodadek, T., and Chiang, C.-M. (2016) BRD4 phosphorylation regulates HPV E2-mediated viral transcription, origin replication, and cellular MMP-9 expression. Cell Reports 16:1733-1748. He, C. and Bonasio, R. (2017) A cut above. Elife 6. pii: e25000. Meers, M.P., Bryson, T.D., Henikoff, J.G., and Henikoff, S. (2019) Improved CUT&RUN chromatin profiling tools. Elife 8. pii: e46314. Wu S.-Y., Lee, C.F., Lai, H.T., Yu, C.T., Lee, J.E., Zuo, H., Tsai, S.Y., Tsai, M.J., Ge, K., Wan, Y., and Chiang, C.-M. (2020) Opposing functions of BRD4 isoforms in breast cancer. Mol. Cell 78: 1114-1132. Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, Liu Z, Mancini MA, Li W. (2021) Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation. Mol. Cell 81: 3368-3385.

教學進度Course Schedule

2023/08/21 The General Transcription Machinery and Core Promoter Elements I The General Transcription Machinery and Core Promoter Elements I 許凱文(Hsu, Kai-Wen)
2023/08/28 The General Transcription Machinery and Core Promoter Elements II The General Transcription Machinery and Core Promoter Elements II 許凱文(Hsu, Kai-Wen)
2023/09/04 Transcription Cofactors I Transcription Cofactors I 許凱文(Hsu, Kai-Wen)
2023/09/11 Transcription Cofactors II Transcription Cofactors II 許凱文(Hsu, Kai-Wen)
2023/09/18 Transcription Cofactors III Transcription Cofactors III 許凱文(Hsu, Kai-Wen)
2023/09/25 Sequence-Specific Transcription Factors, Enhancers, and Insulators I Sequence-Specific Transcription Factors, Enhancers, and Insulators I 許凱文(Hsu, Kai-Wen)
2023/10/02 Sequence-Specific Transcription Factors, Enhancers, and Insulators II Sequence-Specific Transcription Factors, Enhancers, and Insulators II 許凱文(Hsu, Kai-Wen)
2023/10/09 Transcriptional modification Transcriptional modification 許凱文(Hsu, Kai-Wen)
2023/10/16 Mid-term report Mid-term report 許凱文(Hsu, Kai-Wen)
2023/10/23 Chromatin-Dependent Transcription & Epigenetic Control of Gene Expression I Chromatin-Dependent Transcription & Epigenetic Control of Gene Expression I 許凱文(Hsu, Kai-Wen)
2023/10/30 Chromatin-Dependent Transcription & Epigenetic Control of Gene Expression II Chromatin-Dependent Transcription & Epigenetic Control of Gene Expression II 許凱文(Hsu, Kai-Wen)
2023/11/06 Post-Translational Modification & Cancer I Post-Translational Modification & Cancer I 許凱文(Hsu, Kai-Wen)
2023/11/13 Post-Translational Modification & Cancer II Post-Translational Modification & Cancer II 許凱文(Hsu, Kai-Wen)
2023/11/20 Viral Latency and Gene Reactivation I Viral Latency and Gene Reactivation I 許凱文(Hsu, Kai-Wen)
2023/11/27 Viral Latency and Gene Reactivation II Viral Latency and Gene Reactivation II 許凱文(Hsu, Kai-Wen)
2023/12/04 Chromatin Dynamics I Chromatin Dynamics I 許凱文(Hsu, Kai-Wen)
2023/12/11 Chromatin Dynamics II Chromatin Dynamics II 許凱文(Hsu, Kai-Wen)
2023/12/18 Final term report Final term report 許凱文(Hsu, Kai-Wen)