本課程旨在讓研究生於論文研究開始前,能夠有系統的學習各種生物技術的原理及其操作步驟.教授的老師將就其專長之技術,作深入淺出的介紹.本課程除了讓學生熟悉各項實驗技術的操作原理及技巧外,也希望將老師之經驗傳承給學生.希望有助於學生未來論文的研究.
Before starting their thesis, students will learn various biotechniques, from theory to detail experimental steps, mainly through the instruction of the teachers that know these techniques very well. Every teacher will do their best to teach students and hope that this class will help students a lot with their thesis.
先修科目Prerequisites
無
none
教學方式Teaching Methods
講課
Lecturing
評量方式Assessment
其它
Others
作業及報告
參考書目Reference
(1) Molecular Cloning-by Joseph Sambrook and David W. Russell
(2) Current Protocols in Cell Biology-John Wiley & Sons. Inc.
教學進度Course Schedule
2019/09/12 蛋白質測定和西方式墨點法
Protein determination and Western blotting
黃文欽(Huang,Wen-Chin) 2019/09/19 流式細胞儀
Flow cytometry
陳惠珍(Hui-Chen Chen) 2019/09/26 CRISPR/Cas9在基礎研究及臨床上的應用
Application of CRISPR/Cas9 in basic research and clinics
吳恒祥(Wu, Heng-Hsiung) 2019/10/03 基因選殖
Gene cloning
蘇文琪(Wen-Chi Su) 2019/10/10 免疫沉澱法
Immunoprecipitation
陳慧婕(Hui-Jye Chen) 2019/10/17 核酸測定與定量聚合酵素鏈鎖反應
Nucleic acid detection and quantitative PCR
鄭如茜(Ju Chien Cheng) 2019/10/24 免疫螢光染色法
Immunofluorescent staining
陳慧婕(Hui-Jye Chen) 2019/10/31 去氧核糖核酸(DNA)的轉殖
DNA transfection
陳慧婕(Hui-Jye Chen) 2019/11/07 期中考週
Midterm examination
陳慧婕(Hui-Jye Chen) 2019/11/14 核糖核酸干擾技術
RNAi technology
石志榮(Chih-Rong, Shyr) 2019/11/21 利用細胞和小鼠模型研究癌症
Cell and mouse models for cancer research
石志榮(Chih-Rong, Shyr) 2019/11/28 組織工程於生物醫學之應用
Application of tissue engineering in biomedical sciences
洪慧珊(Huey-Shan Hung) 2019/12/05 細胞週期;細胞生長
Cell cycle; cell proliferation
謝佳宏(Chia-Hung Hsieh) 2019/12/12 體內與體外螢光影像分析
In vitro and in vivo fluorescence imaging analysis
謝佳宏(Chia-Hung Hsieh) 2019/12/19 體細胞核轉置的原理與其應用
Principle and applications of somatic cell nuclear transfer technology in biomedical research
朱志成(Jyh-Cherng Ju) 2019/12/26 蛋白質體學及其應用
Proteomics and the applications in biomedical research
鄭如茜(Ju Chien Cheng) 2020/01/02 基因轉殖鼠及基因剔除鼠技術
Transgenic/knockout mice
劉詩平(Shih-Ping Liu) 2020/01/09 期末考週
Final examination
陳慧婕(Hui-Jye Chen)