介紹腫瘤細胞生物學的知識與實驗技術，並輔以研究論文討論加深學生印象，以達成觀念、學理、儀器、技術等之建立，使學生對科學研究有具體認識，期望能使研究生對腫瘤細胞生物學的知識與實驗技術有全面性的了解並能應用這些知識於個人研究、專題與論文。
The present course will discuss the principle of biotechniques specifically applied in the current research of tumor cell biology, biochemistry, molecular biology and oncology. It consists of basic and advanced gene engineering, analysis of micro/macromolecules and tumor cell behavior, and establishment of animal models. The omics revolutions as well as nanotechnologies are also included. Lectures, activity and hands-on practices will allow students to familiarize with the contents, methods, and instruments as well as to build their ability in scientific research design, performance and application.
先修科目Prerequisites
None
None
教學方式Teaching Methods
同步教學(使用虛擬教室如：Moodle同步教室、Google Meet、Microsoft Classroom等)
Synchronous Online Learning
講課
Lecturing
小組討論、腦力激盪(如案例討論、電影欣賞等)
Group discussion, Brain storming, e.g., Case study, Event discussion, Movie review
一分鐘回饋、其他
One-minute feedback, Others
評量方式Assessment
期末報告
Final report
出席狀況
Class attendance
課堂參與與表現
Class involvement
參考書目Reference
1. Introduction to Biotechnology , William J. Thieman and Michael A. Palladino (Ed.), 3rd Edition, 2009
2. Nature Biotechnology, Journal
3. Nature Protocols, Journal
教學進度Course Schedule
2022/09/15 導論
General Introduction
黃文欽(Huang,Wen-Chin) 2022/09/22 分子生物學和生化學（I）： 去氧核糖核酸分析
Molecular Biology and Biochemistry (I): DNA
黃文欽(Huang,Wen-Chin) 2022/09/29 分子生物學和生化學（II）： 核糖核酸分析
Molecular Biology and Biochemistry (II): RNA Analysis
黃文欽(Huang,Wen-Chin) 2022/10/06 分子生物學和生化學（III）： 蛋白質分析
Molecular Biology and Biochemistry (III): Protein Analysis
黃文欽(Huang,Wen-Chin) 2022/10/13 分子生物學和生化學（IV）： 基因選殖
Molecular Biology and Biochemistry (IV): Gene Cloning
黃文欽(Huang,Wen-Chin) 2022/10/20 腫瘤細胞生物學（I）：腫瘤細胞培養
Tumor Cell Biology (I): Tumor Cell Culture
黃文欽(Huang,Wen-Chin) 2022/10/27 腫瘤細胞生物學（II）：腫瘤細胞生長分析
Tumor Cell Biology (II): Tumor Cell Proliferation
黃文欽(Huang,Wen-Chin) 2022/11/03 腫瘤細胞生物學（III）：腫瘤細胞死亡分析
Tumor Cell Biology (III): Tumor Cell Death
黃文欽(Huang,Wen-Chin) 2022/11/10 期中考
Mid-examine
黃文欽(Huang,Wen-Chin) 2022/11/17 腫瘤細胞生物學（IV）：腫瘤細胞移動及侵入分析
Tumor Cell Biology (IV): Tumor Cell Migration and Invasion
黃文欽(Huang,Wen-Chin) 2022/11/24 腫瘤生物學（I）：體外腫瘤發生的檢測與分析
Tumor Biology (I): In vitro Tumorigenecity Assay
黃文欽(Huang,Wen-Chin) 2022/12/01 腫瘤生物學（II）：體內腫瘤發生--動物模式的建立
Tumor Biology (II): In vivo Tumorigenecity Assay-- Animal Models
黃文欽(Huang,Wen-Chin) 2022/12/08 腫瘤生物學（III）：分子影像
Tumor Biology (III): Molecular Imaging
黃文欽(Huang,Wen-Chin) 2022/12/15 基因體學分析
Analysis of Genomics
黃文欽(Huang,Wen-Chin) 2022/12/22 蛋白質體學分析
Analysis of Proteomics
黃文欽(Huang,Wen-Chin) 2022/12/29 代謝質體學分析
Analysis of Metabolomics
黃文欽(Huang,Wen-Chin) 2023/01/05 奈米科技
Nanotechnology
黃文欽(Huang,Wen-Chin) 2023/01/12 期末考
Final examine
黃文欽(Huang,Wen-Chin)