Introduction of nucleotides and nucleic acid: some basics, nucleic acid structure, chemistry and other functions
教學目標Course Objectives
本課程主要介紹的DNA/RNA的分子開始介紹，其蛋白質之生合成，最後再談及相關的實驗技術原理與應用。
Introduction of the DNA / RNA molecules, the protein biosynthesis, the final return to the relevant experimental techniques principle and application.
先修科目Prerequisites
無
no
教學方式Teaching Methods
案例或故事討論
Case study or event discussion
講課
Lecturing
小組討論
Group discussion
學生上台報告
Oral presentation
評量方式Assessment
期中報告
Midterm report
期末報告
Final report
期中考試30%
期末考試40%
平時成績30%
參考書目Reference
1.Molecular Biology (2001, 2nd edition.) Robert F. Weaver。McGraw-Hill出版。
2.Molecular Cell Biology (2003, 5th edition.) Harvey F. Lodish, Arnold Berk, Paul Matsudaira, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, S. Lawrence Zipursky, James Darnell。W H Freeman出版。
3.Genes VIII (2003, 8th edition.) Benjamin Lewin。Prentice Hall出版。
教學進度Course Schedule
2017/09/29 分子生物學簡介和染色質的結構
Central dogma of molecular biology & structure of chromatin
黃冠中(Huang Guan-Jhong) 2017/10/06 染色體的基因功能
Definition of a functional gene on a chromosome
黃冠中(Huang Guan-Jhong) 2017/10/13 轉錄調控基因表現
Regulation of gene expression at the transcriptional level
黃冠中(Huang Guan-Jhong) 2017/10/20 DNA和蛋白質的相互作用
DNA and protein interactions
黃冠中(Huang Guan-Jhong) 2017/10/27 RNA剪接，RNA編輯
RNA splicing, RNA editing.
黃冠中(Huang Guan-Jhong) 2017/11/03 轉錄調控基因表現
Regulation of gene expression at the post- transcriptional level
黃冠中(Huang Guan-Jhong) 2017/11/10 轉錄調控基因表現II
Regulation of gene expression at the post- transcriptional level II
黃冠中(Huang Guan-Jhong) 2017/11/17 rRNA基因，tRNA基因，與核糖體進入位點
rRNA genes, tRNA genes, and ribosomal entry site
黃冠中(Huang Guan-Jhong) 2017/11/24 期中考
test
李孟修(Lee, Meng-Shiou) 2017/12/01 反義 RNA干擾
anti-sense RNA interference.
李孟修(Lee, Meng-Shiou) 2017/12/08 調控基因表達在翻譯後水平
Regulation of gene expression at the post- translational level
李孟修(Lee, Meng-Shiou) 2017/12/15 蛋白質折疊，二硫鍵的形成，分裂
protein folding, disulfide bond formation, and cleavage
張文德(Wen-Te Chang) 2017/12/22 糖基化和磷酸化。
glycosylation and phosphorylation.
張文德(Wen-Te Chang) 2017/12/29 RNA聚合脢和逆轉錄脢
RNA dependent RNA polymerase and reverse transcriptase.
游邦照(Bang-Jau You) 2018/01/05 細胞內防禦：限制性內切脢
原則的DNA分克隆。
Intra cellular defense: restriction enzymes.
Principles of DNA sub-cloning.
游邦照(Bang-Jau You) 2018/01/12 在細胞的DNA組成：限制性片段長度多態性，RAPD分析，和SNP。
DNA composition in cells: RFLP, RAPD, and SNP.
林民昆(Lin, Ming-Kuem) 2018/01/19 期末考
Test
林民昆(Lin, Ming-Kuem)