植物組織培養（Plant Tissue Culture）為生物科技的基礎，故本課程將以深入淺出的方式，介紹植物組織培養的基本原理、方法、儀器、和應用，內容包括器官、組織、細胞等培殖體之建立、體胚之誘導、癒合組織的誘導、細胞懸浮培養、二次代謝產物、種源保存、大量繁殖、馴化及遺傳工程上的應用等。配合實務(實驗課的實際操作)與教學，引導學生對植物組織培養能有完整的認識，從而有助於學術生涯之規劃。
Plant tissue culture as the basis for biotechnology, it will be easy to understand the way this course is to introduce the basic principles of plant tissue culture, methods, instruments, and applications, including organs, tissues, cells and other body in the rearing of the establishment, somatic embryos induction, the induction of callus, cell suspension culture, secondary metabolites, provenance preservation, a lot of breeding, domestication and genetic engineering on the application. with practical (hands-on experiment course) and teaching, guide students on plant tissue culture can have a complete understanding, thus contributing to academic career planning.
先修科目Prerequisites
藥用植物學，需同時修植物組織培養正課。
Pharmaceutical botany, Need to take the main course of plant tissue culture.
教學方式Teaching Methods
非同步教學(主要提供線上影音教材，透過線上教學平台進行作業及討論)
Asynchronous Online Learning
講課
Lecturing
學生實作
Student hands-on practice
習題練習、書面報告
Exercise, Written report
觀察與資料收集、採訪
Observation and data collection, Interview
學生創新、創意活動
Innovation, creative activities
評量方式Assessment
期末筆試
Final exam
專題報告
Project report
實作作品與反思
Practical work and introspection
出席狀況
Class attendance
課堂參與與表現
Class involvement
心得與反思報告
Learning gain and introspection report
其它
Others
平時表現（10％）、實驗紀錄簿及報告（40％）、期中考及操作成果（20％）、期末考（30％）
2022/09/15 組織培養之實驗概念，培養器皿之清洗、消毒、無菌操作及儀器設備之安全使用。
The concept of plant tissue culture experiments, the culture vessel cleaning, disinfection, aseptic operation and the safe use of equipment.
郭昭麟(Kuo Chao-Lin) 2022/09/29 組織培養之培養基儲存液及植物生長調節劑儲存液之製備及注意事項
The storage of liquid tissue culture medium and plant growth regulators, preparation and storage of liquid notes.
郭昭麟(Kuo Chao-Lin) 2022/10/13 組織培養之培養基之配製與,種子無菌播種之操作
Plant tissue culture medium for the preparation and seed sowing of aseptic operation
郭昭麟(Kuo Chao-Lin) 2022/10/27 實驗紀錄簿繕寫相關事項
Experimental record book write related matters
郭昭麟(Kuo Chao-Lin) 2022/11/10 組織培養之培養基的配製與蕨類胞子無菌播種之操作
Plant tissue culture medium fern spore preparation and sowing of the operation of aseptic
郭昭麟(Kuo Chao-Lin) 2022/11/24 組織培養之培養基的配製與培殖體的建立
Preparation of plant tissue culture medium and in the rearing of body build
郭昭麟(Kuo Chao-Lin)蔡新聲 2022/12/08 組織培養之癒合組織誘導與繼代（一）
Plant tissue culture of callus induction and subculture(Ⅰ)
郭昭麟(Kuo Chao-Lin) 2022/12/22 組織培養之癒合組織誘導與繼代（二）
Plant tissue culture of callus induction and subculture(Ⅱ)
郭昭麟(Kuo Chao-Lin) 2023/01/05 期末考
Final-term examination
郭昭麟(Kuo Chao-Lin)