Recombinant DNA technology
Genomics
Proteomics
Metabolomics
Nanotechnology
Animal Models
教學目標Course Objectives
本課程的目的在讓同學對現代的生物科技有基本的認識，內容將包括基本的基因操作及後基因體時代的生物科技。
There has been rapid progress in recombinant DNA technology over the last few years, particularly in the area of large-scale biology (genomics and related disciplines). The increasing integration between gene manipulation and genomics is embraced in this course. This important course consists of basic and advanced gene-manipulation techniques, genome analysis, genomics, transcriptomics, proteomics, and metabolomics, as well as the applications of these fascinating technologies.
先修科目Prerequisites
生物化學
Biochemistry
教學方式Teaching Methods
非同步教學(主要提供線上影音教材，透過線上教學平台進行作業及討論)
Asynchronous Online Learning
講課
Lecturing
學生實作
Student hands-on practice
習題練習、書面報告
Exercise, Written report
評量方式Assessment
期中筆試
Midterm exam
期末筆試
Final exam
出席狀況
Class attendance
課堂參與與表現
Class involvement
心得與反思報告
Learning gain and introspection report
影片瀏覽(10%)、線上隨堂測驗(10%)、期中筆試(35%)、期末筆試(35%)、面授出席(10%)
參考書目Reference
1. Principles of Gene Manipulation and Genomics. SB Primrose and R.M. Twyman, 7th edition, Blackwell, 2006
2. Molecular Biotechnology: Principles and Applications of Recombinant DNA. Bernard R. Glick, Jack J. Pasternak. 5th edition, ASM Press. 2017
3. Topic related references
教學進度Course Schedule
2023/09/14 課程介紹、基因操作與後基因體時代
Gene manipulation in the post-genomics era
林振文(Lin Cheng Wen) 2023/09/21 基因操作基本技術的介紹
Basic techniques: blotting , gene cloning, PCR, transformation
鄭如茜(Ju Chien Cheng) 2023/09/28 質體構築策略的介紹
Gene-cloning strategies
鄭如茜(Ju Chien Cheng) 2023/10/05 質體功能性篩選及定序方法的介紹
Sequencing technology and functional selection
鄭如茜(Ju Chien Cheng) 2023/10/12 在細菌、酵母菌及動物細胞表現質體的方法
Cloning and expression in bacteria, yeast and animal cells
黃蕙君(HUANG, Huey-Chun) 2023/10/19 動物的基因操作
Genetic manipulation of animals
黃蕙君(HUANG, Huey-Chun) 2023/10/26 基因治療及細胞治療
Gene therapy and cell therapy
黃蕙君(HUANG, Huey-Chun) 2023/11/02 單株抗體與人源化抗體
Preparation and application of monoclonal and humanized antibodies
林孟亮(Meng-Liang Lin) 2023/11/09 期中考
Mid-term Examination
鄭如茜(Ju Chien Cheng) 2023/11/16 基因體的介紹
Genome structure, mapping and sequencing genomes
林振文(Lin Cheng Wen) 2023/11/23 比較基因體學、晶片分析與生物資訊
Comparative genomics and microarray analysis and bioinformatics
林瑜姿(Lin,Yu-Tzu) 2023/11/30 蛋白的表現、純化、分析與鑑定
Expression, purification, analysis and characterization of proteins
林振文(Lin Cheng Wen) 2023/12/07 蛋白結構與交互作用
Analysis protein structures and protein interactions
林振文(Lin Cheng Wen) 2023/12/14 代謝體學
Metabolomics
林瑜姿(Lin,Yu-Tzu) 2023/12/21 體學應用討論與實作
Practice and discussion of "Omic" application
林瑜姿(Lin,Yu-Tzu) 2023/12/28 合成生物學
Synthetic Biology
姜中人(Chung-Jen Chiang) 2024/01/04 奈米生物科技
Nanobiotechnology
姜中人(Chung-Jen Chiang) 2024/01/11 期末考
Final Examination
鄭如茜(Ju Chien Cheng)